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Gelatinase B (MMP-9) gene silencing does not affect murine preadipocyte differentiation.

Authors
  • Bauters, Dries
  • Van Hul, Matthias
  • Lijnen, Henri Roger
Type
Published Article
Journal
Adipocyte
Publication Date
Jan 01, 2014
Volume
3
Issue
1
Pages
50–53
Identifiers
DOI: 10.4161/adip.26966
PMID: 24575369
Source
Medline
Keywords
License
Unknown

Abstract

Growth of adipose tissue involves differentiation of preadipocytes into mature lipid-containing adipocytes. The matrix metalloproteinases (MMPs) are known regulators of adipose tissue biology, and previous studies suggested a key role for gelatinase B (MMP-9) in adipogenesis. In the present study we have evaluated a potential functional role of MMP-9 by performing gene silencing in 3T3-F442A preadipocytes. At the end of a 12-day differentiation period, no significant differences were observed between MMP-9 knockdown cells and the control cells with respect to intracytoplasmatic lipid content, or expression of the adipogenic markers aP2, PPARγ, Lpl, and adiponectin, or of the preadipocyte marker Pref-1. Thus, in vitro differentiation of 3T3-F442A preadipocytes into mature adipocytes is not significantly affected by the loss of MMP-9.

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