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Gd3+-nanoparticle-enhanced multivalent biosensing that combines magnetic relaxation switching and magnetic separation.

Authors
  • Xianyu, Yunlei1
  • Dong, Yongzhen2
  • Zhang, Zhuo2
  • Wang, Zhanhui3
  • Yu, Wenbo3
  • Wang, Zhilong2
  • Chen, Yiping4
  • 1 College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, Zhejiang, China; Fuli Institute of Food Science, Zhejiang University, Hangzhou, 310058, Zhejiang, China; Ningbo Research Institute, Zhejiang University, Ningbo, 315100, Zhejiang, China. , (China)
  • 2 College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, Wuhan, China. , (China)
  • 3 Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, and Beijing Laboratory for Food Quality and Safety, Beijing, 100193, China. , (China)
  • 4 College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, Wuhan, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Biosensors & bioelectronics
Publication Date
May 01, 2020
Volume
155
Pages
112106–112106
Identifiers
DOI: 10.1016/j.bios.2020.112106
PMID: 32090877
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

In this work, we developed a multivalent magnetic biosensing strategy by integrating magnetic separation and magnetic relaxation switching (MRS) where Gd3+-loaded magnetic nanoparticles acted as the probe. As a transition metal ion, Gd3+ has multiple unpaired electrons in the d-orbitals that can induce a strong fluctuating magnetic field and thus can reduce the transverse relaxation time (T2), contributing to a strong magnetic signal. By loading Gd3+ onto magnetic nanoparticles, we prepared a multivalent magnetic probe that combined magnetic separation and MRS for the signal readout. This multivalent sensing technique simplified the procedures and greatly enhanced the detection sensitivity of conventional MRS assays. A sensitive detection of ractopamine in real samples has been demonstrated with this multivalent sensing technique. The magnetic probe enabled the detection of ractopamine in a linear range from 0.1 to 100 ng/mL and the limit of detection was 20 pg/mL, a 25-fold enhancement in the sensitivity compared with conventional MRS assays. This Gd3+-nanoparticle-mediated MRS biosensor is a potential magnetic platform to detect trace levels of targets in complex samples. Copyright © 2020 Elsevier B.V. All rights reserved.

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