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Gas-liquid chromatography of ethyl ester artifacts formed during the preparation of fatty acid methyl esters.

Authors
Type
Published Article
Journal
Journal of lipid research
Publication Date
Volume
17
Issue
4
Pages
431–432
Identifiers
PMID: 950505
Source
Medline
License
Unknown

Abstract

Ethanol is typically used as a stabilizer in chloroform. Failure to remove this ethanol from the chloroform used in the extraction of lipids leads subsequently to the formation of ethyl ester artifacts during the preparation of methyl esters by a commonly employed transesterification procedure. Depending on the conditions and phases used during gas-liquid chromatography, the ethyl esters may be resolved from the corresponding methyl esters. The resulting chromatograms contain extraneous peaks and may be incorrectly identified.

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