Gas-liquid chromatography of ethyl ester artifacts formed during the preparation of fatty acid methyl esters.
- Published Article
Journal of lipid research
- Publication Date
Jul 01, 1976
Ethanol is typically used as a stabilizer in chloroform. Failure to remove this ethanol from the chloroform used in the extraction of lipids leads subsequently to the formation of ethyl ester artifacts during the preparation of methyl esters by a commonly employed transesterification procedure. Depending on the conditions and phases used during gas-liquid chromatography, the ethyl esters may be resolved from the corresponding methyl esters. The resulting chromatograms contain extraneous peaks and may be incorrectly identified.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/950505