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Gas chromatographic determination of alkyl lysophospholipids after solid-phase extraction from cell culture media.

Authors
  • Coene, J
  • Van den Eeckhout, E
  • Herdewijn, P
  • Sandra, P
Type
Published Article
Journal
Journal of chromatography
Publication Date
Jan 29, 1993
Volume
612
Issue
1
Pages
21–26
Identifiers
PMID: 8454698
Source
Medline
License
Unknown

Abstract

The gas chromatographic determination of 1-O-octadecyl-2-O-methyl-DL-glycero-3-phosphorylcholine (Et-18-OMe), an anti-invasive alkyl lysophospholipid, in cell culture media is described. Sample clean-up was performed by solid-phase extraction on a weak cation-exchange column of the CBA type (carboxylic acid). For quantitation, the structural analogue Et-16-OMe as the internal standard was used after derivatization with trimethylsilyl bromide. The described method was free of interferences in cell culture media. The overall precision for twenty determinations was 14.99%.

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