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gamma-Radiolysis of DNA in oxygenated aqueous solutions: alterations at the sugar moiety.

Authors
  • Isildar, M
  • Schuchmann, M N
  • Schulte-Frohlinde, D
  • von Sonntag, C
Type
Published Article
Journal
International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine
Publisher
Informa UK (Taylor & Francis)
Publication Date
Oct 01, 1981
Volume
40
Issue
4
Pages
347–354
Identifiers
PMID: 6975249
Source
Medline
License
Unknown

Abstract

On gamma-irradiation of DNA in N2O/O2-saturated aqueous solutions alterations at the sugar moiety are observed. In the present study three new lesions were recognized: (i) 2-deoxytetrodialdose bound via a phosphoric acid ester linkage to a (broken) DNA strand, (ii) 2-deoxypentos-4-ulose bound to DNA via one (or two?) phosphoric acid ester linkage(s), and (iii) 2-deoxy-D-erythro-pentose bound to DNA via two phosphoric acid ester linkages. Lesion (i) is directly connected with a DNA strand break. Lesion (ii) might be related to a DNA strand break if bound via only one phosphoric acid ester linkage, or has to be considered as an alkali-labile site if bound via two phosphoric acid ester linkages. Lesion (iii) results from base damage, when the damaged base is hydrolysed from the sugar. This lesion is an alkali-labile site which turns into a strand break on alkali treatment. Attempts have been made to quantify these lesions. A lower limit of sugar damage (including lesions observed in preceding studies, but not lesion (iii) of G = 0.25 has been estimated.

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