The game of touch rugby is considered safe, yielding minimal acute musculoskeletal injuries. This study profiled the prevalence of acute touch rugby musculoskeletal injury among female adolescent non-elite, recreational players over a 6 month period (February-July 2014). Data were collected from 76 high school players participating, in the A League in the Upper High Way Area of Kwa Zulu-Natal Province, South Africa, via voluntary parental informed consent and player ascent. Players completed a self-report questionnaire probing the nature and mechanism of acute musculoskeletal injuries. Probability was set at p 0.05. Forty-nine players sustained acute musculoskeletal injuries with the ankle (33.3%) and knee (28.8%) being most prevalent (p<0.001). The Link player position indicated most injuries (p<0.0001). The mechanisms producing the musculoskeletal injuries were rapid, rotational movement (33.3%) and poor landing (26.6%) (p<0.05). Female adolescent touch rugby players experience a high prevalence of acute musculoskeletal knee and ankle injuries.Keywords: Touch rugby, musculoskeletal injury, female adolescents.