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Galectin-12 is Involved in Corn Silk-Induced Anti-Adipogenesis and Anti-Obesity Effects.

Authors
  • Hsu, Yu-An1
  • Kuo, Yi-Hsin1
  • Chen, Chih-Sheng2, 3, 4
  • Chen, Ying-Chi5
  • Huang, Chi-Chun6
  • Chang, Ching-Yao7
  • Lin, Chao-Jen8, 9
  • Lin, Cheng-Wen10
  • Lin, Hui-Ju11, 12
  • Liu, Fu-Tong6, 12
  • Wan, Lei1, 13, 14, 7
  • 1 * School of Chinese Medicine, China Medical University, Taichung, Taiwan. , (China)
  • 2 † Graduate Institute of Chinese Medicine, China Medical University, Taichung, Taiwan. , (China)
  • 3 ¶ Division of Chinese Traumatology, China Medical University Hospital, Taichung, Taiwan. , (China)
  • 4 †† Division of Chinese Medicine, Asia University Hospital, Taichung, Taiwan. , (Taiwan)
  • 5 ‡‡ Department of Biomedical Science, Bonn-Rhein-Sieg University of Applied Science, Rheinbach, Germany. , (Germany)
  • 6 §§ Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. , (Taiwan)
  • 7 ¶¶ Department of Biotechnology, Asia University, Taichung, Taiwan. , (Taiwan)
  • 8 ∥∥ Department of Pediatrics, Changhua Christian Children's Hospital, Changhua, Taiwan. , (Taiwan)
  • 9 *** School of Medicine, Chung Shan Medical University, Taichung, Taiwan. , (Taiwan)
  • 10 ‡ Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan. , (China)
  • 11 ∥ Department of Ophthalmology, China Medical University Hospital, Taichung, Taiwan. , (China)
  • 12 ††† Department of Dermatology, University of California, Davis School of Medicine, Sacramento, California, USA.
  • 13 § Research Center for Chinese Medicine & Acupuncture, China Medical University, Taichung, Taiwan. , (China)
  • 14 ** Department of Obstetrics and Gynecology, China Medical University Hospital, Taichung, Taiwan. , (China)
Type
Published Article
Journal
The American journal of Chinese medicine
Publication Date
Jan 01, 2018
Volume
46
Issue
5
Pages
1045–1063
Identifiers
DOI: 10.1142/S0192415X18500544
PMID: 29976086
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Obesity is a significant risk factor for various diseases. It is a clinical condition caused by the excessive accumulation of fat, which has a negative impact on human health. Galactin-12 is an adipocyte-expressed protein and possesses adipocyte-inducing activity. We investigated the expression level of candidate proteins involved in galactin-12-mediated adipocyte differentiation pathway. We performed a high-throughput screening assay to monitor galectin-12 promoter activity using 105 traditional Chinese herbs. Corn silk extract and [Formula: see text]-sitosterol reduced the expression of galactin-12 promoter in 3T3-L1 cells. In addition, corn silk extract and [Formula: see text]-sitosterol decreased the level of lipid droplets and downregulated the gene and protein expression level of C/EBP[Formula: see text], C/EBP[Formula: see text], PPAR[Formula: see text], Ap2, and adipsin in 3T3-L1 pre-adipocytes via AKT and ERK1/2 inhibition. In vivo study with the oral administration of corn silk extract and [Formula: see text]-sitosterol in a mouse model showed a significant weight reduction and decrease in adipocytes in several organs such as the liver and adipose tissue. Taken together, corn silk extract and [Formula: see text]-sitosterol may effectively reduce pre-adipocyte differentiation by inhibiting galectin-12 activity and exerting anti-obesity effects. These findings highlight the potential use of corn silk extract and [Formula: see text]-sitosterol as potential candidates for the prevention and treatment of obesity.

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