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Gain-of-function human STAT1 mutations impair IL-17 immunity and underlie chronic mucocutaneous candidiasis.

Authors
  • Luyan Liu
  • Satoshi Okada
  • Xiao-Fei Kong
  • Alexandra Y Kreins
  • Sophie Cypowyj
  • Avinash Abhyankar
  • Julie Toubiana
  • Yuval Itan
  • Magali Audry
  • Patrick Nitschke
  • Cécile Masson
  • Beata Toth
  • Jérome Flatot
  • Mélanie Migaud
  • Maya Chrabieh
  • Tatiana Kochetkov
  • Alexandre Bolze
  • Alessandro Borghesi
  • Antoine Toulon
  • Julia Hiller
  • And 46 more
Identifiers
DOI: 10.1084/jem.20110958
Source
CdV-UPMC
License
Unknown

Abstract

Chronic mucocutaneous candidiasis disease (CMCD) may be caused by autosomal dominant (AD) IL-17F deficiency or autosomal recessive (AR) IL-17RA deficiency. Here, using whole-exome sequencing, we identified heterozygous germline mutations in STAT1 in 47 patients from 20 kindreds with AD CMCD. Previously described heterozygous STAT1 mutant alleles are loss-of-function and cause AD predisposition to mycobacterial disease caused by impaired STAT1-dependent cellular responses to IFN-γ. Other loss-of-function STAT1 alleles cause AR predisposition to intracellular bacterial and viral diseases, caused by impaired STAT1-dependent responses to IFN-α/β, IFN-γ, IFN-λ, and IL-27. In contrast, the 12 AD CMCD-inducing STAT1 mutant alleles described here are gain-of-function and increase STAT1-dependent cellular responses to these cytokines, and to cytokines that predominantly activate STAT3, such as IL-6 and IL-21. All of these mutations affect the coiled-coil domain and impair the nuclear dephosphorylation of activated STAT1, accounting for their gain-of-function and dominance. Stronger cellular responses to the STAT1-dependent IL-17 inhibitors IFN-α/β, IFN-γ, and IL-27, and stronger STAT1 activation in response to the STAT3-dependent IL-17 inducers IL-6 and IL-21, hinder the development of T cells producing IL-17A, IL-17F, and IL-22. Gain-of-function STAT1 alleles therefore cause AD CMCD by impairing IL-17 immunity.

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