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GABARAPL1 (GEC1) associates with autophagic vesicles.

Authors
  • Chakrama, Fatima Zahra
  • Seguin-Py, Stéphanie
  • Le Grand, Jaclyn Nicole
  • Fraichard, Annick
  • Delage-Mourroux, Régis
  • Despouy, Gilles
  • Perez, Valérie
  • Jouvenot, Michèle
  • Boyer-Guittaut, Michaël
Type
Published Article
Journal
Autophagy
Publisher
Landes Bioscience
Publication Date
May 01, 2010
Volume
6
Issue
4
Pages
495–505
Identifiers
DOI: 10.4161/auto.6.4.11819
PMID: 20404487
Source
Medline
License
Unknown

Abstract

Gabarapl1 (gec1) was first described as an estrogen regulated gene which shares a high sequence homology with the gabarap gene. We previously demonstrated that GABARAPL1, like GABARAP, interacts with the GABAA receptor and tubulin and promotes tubulin polymerization. Previous work has demonstrated that the GABARAP family members (GABARAP, LC3, GATE-16 and Atg8) are not only involved in the transport of proteins or vesicles but are also implicated in various mechanisms such as autophagy, cell death, cell proliferation and tumor progression. We therefore asked whether GABARAPL1 might also play a role in autophagy. First, we showed that GABARAPL1 is cleaved at glycine 116, a residue which is conserved in other members of the family. We also demonstrated that GABARAPL1 is linked to phospholipids, delipidated by Atg4B, associated with intracellular membranes and accumulated in intracellular vesicles after inhibition of lysosomal activity. Finally, we showed that GABARAPL1 partially colocalizes with LC3 or Lysotracker green in intracellular vesicles. Taken together, our results demonstrate that GABARAPL1 associates with autophagic vesicles.

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