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Fusion of small unilamellar vesicles onto laterally mixed self-assembled monolayers of thiolipopeptides.

Authors
  • Baumgart, T
  • Kreiter, M
  • Lauer, H
  • Naumann, R
  • Jung, G
  • Jonczyk, A
  • Offenhäusser, A
  • Knoll, W
Type
Published Article
Journal
Journal of colloid and interface science
Publication Date
Feb 15, 2003
Volume
258
Issue
2
Pages
298–309
Identifiers
PMID: 12618100
Source
Medline
License
Unknown

Abstract

Monolayers of the thiolipopeptide NH(2)-Cys-Ala-Ser-Ala-Ala-Ser-Ser-Ala-Pro-Ser-Ser-(Myr)Lys(Myr)-OH (III) were formed on gold surfaces by self-assembly, mixed with a lateral spacer of the same peptide composition, NH(2)-Cys-Ala-Ser-Ala-Ala-Ser-Ser-Ala-Pro-Ser-Ser-Lys-OH (I). Different mixing ratios were employed ranging from 0.1 to 1, corresponding to 10-100% thiolipopeptide. These self-assembled monolayers (SAMs) were then exposed to a suspension of liposomes with the aim of forming lipid bilayers as a function of the mixing ratio. A clear optimum with respect to homogeneity and electrical properties of the membranes was obtained in the middle region (0.5) of mixing ratio, as revealed by surface plasmon resonance spectroscopy, impedance spectroscopy, and fluorescence microscopy. The combination of these methods was shown to be a powerful tool, although a true lipid bilayer was not obtained. Instead, vesicle adsorption was shown to be the predominant process, and FRAP (fluorescence recovery after photobleaching) measurements showed that the films were not fluid on the micrometer length scale.

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