Fusion expression of green fluorescent protein and HCV capsid antigene in Escherichia coli cells.
- Published Article
Chinese journal of biotechnology
- Publication Date
Jan 01, 1997
A chimeric gene of the green fluorescent protein (GFP) and hepatitis C virus (HCV) core antigene were constructed and expressed in E. coli cells. The expressed fusion protein was examined by Dot-ELISA and Western blot and the three antigenic determinants were detected. The GFP-Core fusion protein showed not only the striking green fluorescence under natural light but also the HCV antigenic activity. A new method of immunological diagnosis is greatly anticipated in the light of this fusion protein which can be seen as the HCV antigen tagged with the green fluorescent protein.
Report this publication
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
This record was last updated on 07/02/2016 and may not reflect the most current and accurate biomedical/scientific data available from NLM.
The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/9631259