Affordable Access

Fusion expression of green fluorescent protein and HCV capsid antigene in Escherichia coli cells.

Authors
  • Yue, L
  • Qi, Y
  • Lin, H
  • Su, F
  • Deng, X
Type
Published Article
Journal
Chinese journal of biotechnology
Publication Date
Jan 01, 1997
Volume
13
Issue
4
Pages
239–246
Identifiers
PMID: 9631259
Source
Medline
License
Unknown

Abstract

A chimeric gene of the green fluorescent protein (GFP) and hepatitis C virus (HCV) core antigene were constructed and expressed in E. coli cells. The expressed fusion protein was examined by Dot-ELISA and Western blot and the three antigenic determinants were detected. The GFP-Core fusion protein showed not only the striking green fluorescence under natural light but also the HCV antigenic activity. A new method of immunological diagnosis is greatly anticipated in the light of this fusion protein which can be seen as the HCV antigen tagged with the green fluorescent protein.

Report this publication

Statistics

Seen <100 times