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Furin Cleavage Site Is Key to SARS-CoV-2 Pathogenesis

Authors
  • Johnson, Bryan A.1
  • Xie, Xuping2
  • Kalveram, Birte3
  • Lokugamage, Kumari G.1
  • Muruato, Antonio1
  • Zou, Jing2
  • Zhang, Xianwen2
  • Juelich, Terry3
  • Smith, Jennifer K.3
  • Zhang, Lihong3
  • Bopp, Nathen3
  • Schindewolf, Craig1
  • Vu, Michelle1
  • Vanderheiden, Abigail4, 5, 6
  • Swetnam, Daniele2
  • Plante, Jessica A.1
  • Aguilar, Patricia3
  • Plante, Kenneth S.1
  • Lee, Benhur7
  • Weaver, Scott C.1, 8
  • And 9 more
  • 1 Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA
  • 2 Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA
  • 3 Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA
  • 4 Department of Pediatrics, Emory Vaccine Center, Atlanta, GA, USA.
  • 5 Emory University School of Medicine, Atlanta, GA, USA.
  • 6 Yerkes National Primate Research Center, Atlanta, GA, USA
  • 7 Icahn School of Medicine at Mount Sinai, New York, NY, USA
  • 8 Department of Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston, TX, USA
  • 9 Texas Therapeutics Institute, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, USA.
  • 10 Bowie State University, Bowie, MD, USA
Type
Published Article
Journal
bioRxiv
Publisher
Cold Spring Harbor Laboratory
Publication Date
Aug 26, 2020
Identifiers
DOI: 10.1101/2020.08.26.268854
PMID: 32869021
PMCID: PMC7457603
Source
PubMed Central
Keywords
Disciplines
  • Article
License
Unknown

Abstract

A deletion of the furin cleavage site in SARS-CoV-2 amplifies replication in Vero cells, but attenuates replication in respiratory cells and pathogenesis in vivo. Loss of the furin site also reduces susceptibility to neutralization in vitro .

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