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Fungicidal and Cytotoxic Activity of a Capsicum chinense Defensin Expressed by Endothelial Cells

Authors
  • Anaya-López, José L.1
  • López-Meza, Joel E.1
  • Baizabal-Aguirre, Victor M.1
  • Cano-Camacho, Horacio1
  • Ochoa-Zarzosa, Alejandra1
  • 1 Universidad Michoacana de San Nicolás de Hidalgo, Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia, Apdo. Postal 53, Administración Chapultepec, Morelia, Michoacán, C.P. 58262, México , Morelia (Mexico)
Type
Published Article
Journal
Biotechnology Letters
Publisher
Springer-Verlag
Publication Date
Jun 23, 2006
Volume
28
Issue
14
Pages
1101–1108
Identifiers
DOI: 10.1007/s10529-006-9060-4
Source
Springer Nature
Keywords
License
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Abstract

Plant defensins are antimicrobial peptides that exhibit mainly antifungal activity against a broad range of plant fungal pathogens. However, their actions against Candida albicans have not been extensively studied. The mRNA for γ-thionin, a defensin from Capsicum chinense, has been expressed in bovine endothelial cells. The conditioned medium of these cells showed antifungal activity on germ tube formation (60–70% of inhibition) and on the viability of C. albicans (70–80% of inhibition). Additionally, C. albicans was not able to penetrate transfected cells. Conditioned medium from these cells also inhibited the viability (80%) of the human tumor cell line, HeLa.

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