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Functional and Structural Characterization of Melanin from Brevibacillus invocatus Strain IBA

Authors
  • Ammanagi, Avinash1
  • C. T., Shivasharana1
  • R., Krishnaveni2
  • Badiger, Abhijeeth3
  • Ramaraj, Vijayakumar4
  • 1 Department of Studies in Biotechnology and Microbiology, Karnatak University, Dharwad, Karnataka, India , Dharwad (India)
  • 2 Department of Microbiology Vijayanagara Sri Krishna Devaraya University, Ballari, Karnataka, India , Ballari (India)
  • 3 Department of Biotechnology, Sri Dharmasthala Manjunatheshwara College, Ujire, Karnataka, India , Ujire (India)
  • 4 Shilpa Biologicals Private Limited, Dharwad, Karnataka, India , Dharwad (India)
Type
Published Article
Journal
Doklady Biological Sciences
Publisher
Pleiades Publishing
Publication Date
Sep 01, 2021
Volume
500
Issue
1
Pages
159–169
Identifiers
DOI: 10.1134/S001249662105001X
Source
Springer Nature
Keywords
Disciplines
  • Article
License
Yellow

Abstract

AbstractMelanin is a polyphenol or indolic dark brown to black pigment of macromolecules that has a variety of biological functions including UV defence, desiccation, and oxidation. The pigment is classified as a heterogenic polymer. Analytical characterization of melanin can be difficult due to its heterogeneity. In this study, a newly isolated strain of Brevibacillus invocatus strain IBA capable of extracellular melanin production was grown on nutrient agar and the bacteria were molecularly identified. Chemical and physical methods were used to characterize melanin. The solubility of melanin in organic and inorganic solvents was used to characterise it chemically. According to the UV-visible wavelength scan, physical characterization revealed absorption in the UV region 200 to 300 nm, but declining towards the visible region. Functional group identification of extracted melanin was carried out by FTIR with different stretching vibrations at 3226, 2920, 2849, 1628, 1555, 1340 cm–1 and weak absorption bands at 1104 and 1015 cm–1. Structural characterization was carried by SEM of extracted melanin which showed irregular shape and size at different magnifications. The crystallinity of melanin was studied using X-ray crystallography, with a lattice parameter of approximately a = 8.54. The XRD spectrum of the extracted melanin crystallographic pattern revealed peaks at 2θ = 27.32, 31.66, 45.41, 53.84, 53.84, 56.44, 66.18, 73.10, 75.26, and 83.94, which correspond to reflections (111), (200), (220), (311), (222), (400), (331), (420), and (422), respectively. The analytical methods available for melanin analysis are largely complementary, providing detailed knowledge required to draw reliable conclusions about the sample under investigation.

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