Affordable Access

deepdyve-link
Publisher Website

Functional photoacoustic calcium imaging using chlorophosphonazo III in a 3D tumor cell culture

Authors
  • Liu, Wei-Wen1
  • Chen, Sheng-Hong1
  • Li, Pai-Chi1, 1
  • 1 National Taiwan University, Taiwan , (Taiwan)
Type
Published Article
Journal
Biomedical Optics Express
Publisher
The Optical Society
Publication Date
Feb 01, 2021
Volume
12
Issue
2
Pages
1154–1166
Identifiers
DOI: 10.1364/BOE.414602
PMID: 33680564
PMCID: PMC7901334
Source
PubMed Central
License
Unknown

Abstract

This study demonstrates that chlorophosphonazo III (CPZ III) can be used as a contrast agent for photoacoustic calcium imaging. CPZ III can pass across the plasma membrane for labeling intracellular Ca2+ without cytotoxicity. In optical-resolution photoacoustic microscopy (OR-PAM), the photoacoustic (PA) signal intensity was strongly correlated with the presence of CPZ III and Ca2+ at various concentrations. The sensitivity of PA signal reception was enhanced by using an 8 MHz single-element focused ultrasound detector due to their matched frequency characteristics. Differences in the PA signal intensity were successfully found between the core and margin areas of tumorspheres in three-dimensional cell cultures. These findings indicate that CPZ III can serve as a novel PA contrast agent for functional Ca2+ imaging using OR-PAM.

Report this publication

Statistics

Seen <100 times