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Functional diversification upon leader protease domain duplication in the Citrus tristeza virus genome: Role of RNA sequences and the encoded proteins.

Authors
  • Kang, Sung-Hwan1
  • Atallah, Osama O1
  • Sun, Yong-Duo1
  • Folimonova, Svetlana Y2
  • 1 University of Florida, Plant Pathology Department, Gainesville, FL 32611, USA.
  • 2 University of Florida, Plant Pathology Department, Gainesville, FL 32611, USA. Electronic address: [email protected]
Type
Published Article
Journal
Virology
Publisher
Elsevier
Publication Date
Jan 15, 2018
Volume
514
Pages
192–202
Identifiers
DOI: 10.1016/j.virol.2017.11.014
PMID: 29197719
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Viruses from the family Closteroviridae show an example of intra-genome duplications of more than one gene. In addition to the hallmark coat protein gene duplication, several members possess a tandem duplication of papain-like leader proteases. In this study, we demonstrate that domains encoding the L1 and L2 proteases in the Citrus tristeza virus genome underwent a significant functional divergence at the RNA and protein levels. We show that the L1 protease is crucial for viral accumulation and establishment of initial infection, whereas its coding region is vital for virus transport. On the other hand, the second protease is indispensable for virus infection of its natural citrus host, suggesting that L2 has evolved an important adaptive function that mediates virus interaction with the woody host. Copyright © 2017 Elsevier Inc. All rights reserved.

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