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Functional characterization of Vibrio cholerae O1 WbeW enzyme responsible for initial reaction in O antigen biosynthesis

Authors
  • Kim, Chang Sup1
  • Seo, Jeong Hyun2
  • Cha, Hyung Joon3
  • 1 Yeungnam University, School of Chemistry and Biochemistry, Gyeongsan, 712-749, Korea , Gyeongsan (South Korea)
  • 2 Yeungnam University, School of Chemical Engineering, Gyeongsan, 712-749, Korea , Gyeongsan (South Korea)
  • 3 Pohang University of Science and Technology, Department of Chemical Engineering, Pohang, 790-784, Korea , Pohang (South Korea)
Type
Published Article
Journal
Biotechnology and Bioprocess Engineering
Publisher
Springer-Verlag
Publication Date
Nov 01, 2015
Volume
20
Issue
6
Pages
980–987
Identifiers
DOI: 10.1007/s12257-015-0677-9
Source
Springer Nature
Keywords
License
Yellow

Abstract

Vibrio cholerae O1 employs the ATP-binding cassette (ABC) transporter-dependent pathway for O antigen biosynthesis. Different from highly studied Klebsiella pneumoniae and Escherichia coli, it was reported that initial reaction of O antigen biosynthesis in V. cholerae O1 may be involved in WbeW protein, which is predicted to be a galactosyltransferase. In this work, we report expression and characterization of WbeW enzyme. WbeW was expressed as membrane-associated form in E. coli and it was obtained with high purity. The enzyme had a function of transferring Gal-1-P from UDP-Gal to Und-P, implying that initial glycan of O antigen in V. cholerae O1 can be composed of a Gal residue.

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