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Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon.

Authors
  • Tek, Ahmet L
  • Kashihara, Kazunari
  • Murata, Minoru
  • Nagaki, Kiyotaka
Type
Published Article
Journal
Chromosome Research
Publisher
Springer-Verlag
Publication Date
Apr 01, 2010
Volume
18
Issue
3
Pages
337–347
Identifiers
DOI: 10.1007/s10577-010-9119-x
PMID: 20204495
Source
Medline
License
Unknown

Abstract

The centromere as a kinetochore assembly site is fundamental to the partitioning of genetic material during cell division. In order to determine the functional centromeres of soybean, we characterized the soybean centromere-specific histone H3 (GmCENH3) protein and developed an antibody against the N-terminal end. Using this antibody, we cloned centromere-associated DNA sequences by chromatin immunoprecipitation. Our analyses indicate that soybean centromeres are composed of two distinct satellite repeats (GmCent-1 and GmCent-4) and retrotransposon-related sequences (GmCR). The possible allopolyploid origin of the soybean genome is discussed in view of the centromeric satellite sequences present.

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