Affordable Access

Access to the full text

Functional analyses of recombinant mouse hepcidin-1 in cell culture and animal model

Authors
  • Yazdani, Yaghoub1
  • Keyhanvar, Neda2
  • Kalhor, Hamid Reza3
  • Rezaei, Abbas4
  • 1 Infectious Diseases Research Center and Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran , Gorgan (Iran)
  • 2 Golestan University of Medical Sciences, Faculty of Advanced Medical Technologies, Gorgan, Iran , Gorgan (Iran)
  • 3 Sharif University of Technology, Biochemistry Research Laboratory, Department of Chemistry, Tehran, Iran , Tehran (Iran)
  • 4 School of Medicine, Isfahan University of Medical Sciences, Department of Immunology, Isfahan, Iran , Isfahan (Iran)
Type
Published Article
Journal
Biotechnology Letters
Publisher
Springer-Verlag
Publication Date
Apr 04, 2013
Volume
35
Issue
8
Pages
1191–1197
Identifiers
DOI: 10.1007/s10529-013-1198-2
Source
Springer Nature
Keywords
License
Yellow

Abstract

Hepcidin is a peptide hormone that plays an important role in iron metabolism. We have produced a recombinant mouse hepcidin-1 by using baculovirus expression system. Its expression yield was 25 μg/ml when cell culture media were supplemented with a protease inhibitor cocktail. The recombinant mouse hepcidin-1 and synthetic human hepcidin-25 had similar effects on reducing ferroportin expression in J774A cell line and in peritoneal macrophages. However, synthetic human hepcidin-25 was more efficient than recombinant mouse hepcidin-1 in reducing iron concentration in blood circulation (p < 0.01).

Report this publication

Statistics

Seen <100 times