Direct alcohol fuel cells (DAFCs) have been studied over the past decades for applications in portable devices. This article describes state-of-the-art experimental systems for testing and characterizing DAFCs. It starts with an introduction to the general procedures for the design and fabrication of a single cell, with emphasis on the preparation of membrane–electrode assemblies (MEAs) and bipolar plates used for DAFCs. The design of DAFC stacks, including bipolar and monopolar types, is then described. Finally, test loops and the electrochemical instrumentation used for characterizing the electrochemical performance of DAFCs are discussed.