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FspAI, a unique type II restriction endonuclease that recognizes the octanucleotide sequence 5′-RTGC↓GCAY-3′

Authors
  • A. Kesminiene
  • Z. Maneliene
  • J. Vitkute
  • M. Petruyte
  • A. Janulaitis
Publisher
Oxford University Press
Publication Date
Dec 15, 2001
Source
PMC
Keywords
Disciplines
  • Design
License
Unknown

Abstract

A new type II restriction endonuclease designated FspAI has been partially purified from a Flexibacter species Tv-m21K. FspAI recognizes the octanucleotide sequence 5′-RTGC↓GCAY-3′ and cleaves it in the center generating blunt-ended DNA fragments.

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