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From Macrocrystals to Microcrystals: A Strategy for Membrane Protein Serial Crystallography.

Authors
  • Dods, Robert1
  • Båth, Petra1
  • Arnlund, David1
  • Beyerlein, Kenneth R2
  • Nelson, Garrett3
  • Liang, Mengling4
  • Harimoorthy, Rajiv1
  • Berntsen, Peter5
  • Malmerberg, Erik6
  • Johansson, Linda7
  • Andersson, Rebecka1
  • Bosman, Robert1
  • Carbajo, Sergio4
  • Claesson, Elin1
  • Conrad, Chelsie E3
  • Dahl, Peter1
  • Hammarin, Greger1
  • Hunter, Mark S4
  • Li, Chufeng3
  • Lisova, Stella3
  • And 12 more
  • 1 Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden. , (Sweden)
  • 2 Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany. , (Germany)
  • 3 Department of Physics, Arizona State University, Tempe, AZ, USA.
  • 4 Linac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, Menlo Park, CA, USA.
  • 5 Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden; Australian Research Council Centre of Excellence in Advanced Molecular Imaging, La Trobe Institute for Molecular Science, La Trobe University, Melbourne 3086, Australia. , (Australia)
  • 6 Physical Biosciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd, Berkeley, CA 94720, USA.
  • 7 Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden; The Bridge Institute, Department of Chemistry, University of Southern California, Los Angeles, CA 90089-3303, USA. , (Sweden)
  • 8 Department of Photochemistry and Molecular Science, Uppsala University, Uppsala, Sweden. , (Sweden)
  • 9 Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden. Electronic address: [email protected] , (Sweden)
  • 10 Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden. Electronic address: [email protected] , (Sweden)
Type
Published Article
Journal
Structure
Publisher
Elsevier
Publication Date
Sep 05, 2017
Volume
25
Issue
9
Identifiers
DOI: 10.1016/j.str.2017.07.002
PMID: 28781082
Source
Medline
Keywords
License
Unknown

Abstract

Serial protein crystallography was developed at X-ray free-electron lasers (XFELs) and is now also being applied at storage ring facilities. Robust strategies for the growth and optimization of microcrystals are needed to advance the field. Here we illustrate a generic strategy for recovering high-density homogeneous samples of microcrystals starting from conditions known to yield large (macro) crystals of the photosynthetic reaction center of Blastochloris viridis (RCvir). We first crushed these crystals prior to multiple rounds of microseeding. Each cycle of microseeding facilitated improvements in the RCvir serial femtosecond crystallography (SFX) structure from 3.3-Å to 2.4-Å resolution. This approach may allow known crystallization conditions for other proteins to be adapted to exploit novel scientific opportunities created by serial crystallography.

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