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FROGS

Authors
  • Pascal, Géraldine
  • Bernard, Maria
  • Rué, Olivier
  • Escudié, Frédéric
  • Darbot, Vincent
  • Auer, Lucas
Publication Date
Jan 12, 2024
Source
HAL
Keywords
Language
English
License
Unknown
External links

Abstract

FROGS is a CLI workflow designed to produce an ASV count matrix from high depth sequencing amplicon data.FROGS-wrappers allow to add FROGS on a Galaxy instance. (see https://github.com/geraldinepascal/FROGS-wrappers)This workflow is focused on:- User-friendliness with lots of rich graphic outputs and the integration in Galaxy thanks to FROGS-wrappers.- Accuracy with a clustering without global similarity threshold, the management of separated PCRs in the chimera removal step, and the management of multi-affiliations.- Dealing of non overlapping pair of sequences from long amplicon like ITS, or RPB2.- Access to general statistics on microbial communities and differential abundance analysis.- Prediction of functionnal annotation and metabolic pathways.- Speed with fast algorithms parallelisation and easy to use.- Scalability with algorithms designed to support the data growth.

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