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Frequency and expression of genes involved in adhesion and biofilm formation in Staphylococcus aureus strains isolated from periodontal lesions.

Authors
  • Uribe-García, Alina1
  • Paniagua-Contreras, Gloria Luz1
  • Monroy-Pérez, Eric1
  • Bustos-Martínez, Jaime2
  • Hamdan-Partida, Aída2
  • Garzón, Javier3
  • Alanís, Jesica1
  • Quezada, Rosalía1
  • Vaca-Paniagua, Felipe4
  • Vaca, Sergio5
  • 1 FES Iztacala, Universidad Nacional Autónoma de México, Mexico. , (Mexico)
  • 2 Departamento de Atención a la Salud, Universidad Autónoma Metropolitana Xochimilco, Calzada del Hueso 1100, 04960, Ciudad de México, Mexico. , (Mexico)
  • 3 Clínica de Endoperiodontología, FES Iztacala, Universidad Nacional Autónoma de México, Mexico. , (Mexico)
  • 4 Laboratorio Nacional en Salud, Diagnóstico Molecular y Efecto Ambiental en Enfermedades Crónico-Degenerativas, FES Iztacala, Universidad Nacional Autónoma de México México, Mexico; Instituto Nacional de Cancerología, CDMX 14080, Mexico; Unidad de Biomedicina, FES Iztacala, Universidad Nacional Autónoma de México, Mexico. , (Mexico)
  • 5 FES Iztacala, Universidad Nacional Autónoma de México, Mexico. Electronic address: [email protected] , (Mexico)
Type
Published Article
Journal
Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi
Publication Date
Apr 01, 2021
Volume
54
Issue
2
Pages
267–275
Identifiers
DOI: 10.1016/j.jmii.2019.05.010
PMID: 31300301
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The aim of this study was to characterize the Staphylococcus aureus strains isolated from periodontal lesions of patients, to determine the expression of genes involved in cell adhesion upon their infection of human epithelial cells using an in vitro model, its biofilm formation, and its resistance to antibiotics. S. aureus was analysed by PCR, Kirby-Bauer, and pulsed-field gel electrophoresis (PFGE), measuring gene expression by real-time PCR after infection of human cells in vitro. S. aureus was identified in 18.6% (50/268) of the samples. All strains (n = 50) possessed the virulence genes spa (Staphylococcal protein A), coa (coagulase), and icaAB (intercellular adhesin); 96% (n = 48) possessed clfB (clumping factor B), and 88% (n = 44) possessed ebps (elastin-binding protein) and sdrD (serine aspartate repeat protein D). All strains were resistant to methicillin, ampicillin, dicloxacillin, cefotaxime, and penicillin, and were multidrug resistant to 6-12 antibiotics. PFGE analysis showed 37 different pulsed-field types and most strains (60.4%) had a unique pulsed-field type. Twenty-four distinct combinations of virulence genes and antibiotic-resistant phenotypes were identified. Although S. aureus has been considered a transient member of the oral microbiota, our results indicate a high-level expression of virulence genes and multidrug resistance in the strains isolated from periodontal lesions. These strains might complicate the successful treatment of the disease. Copyright © 2019. Published by Elsevier B.V.

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