Affordable Access

deepdyve-link
Publisher Website

Freeze-drying enables homogeneous and stable sample preparation for determination of fecal short-chain fatty acids.

Authors
  • Ueyama, Jun1
  • Oda, Masaya2
  • Hirayama, Masaaki2
  • Sugitate, Kuniyo3
  • Sakui, Norihiro3
  • Hamada, Risa2
  • Ito, Mikako4
  • Saito, Isao2
  • Ohno, Kinji4
  • 1 Department of Pathophysiological Laboratory Sciences, Nagoya University Graduate School of Medicine, 1-1-20 Daikominami, Higashi-ku, Nagoya, 461-8673, Japan. Electronic address: [email protected] , (Japan)
  • 2 Department of Pathophysiological Laboratory Sciences, Nagoya University Graduate School of Medicine, 1-1-20 Daikominami, Higashi-ku, Nagoya, 461-8673, Japan. , (Japan)
  • 3 Agilent Technologies Japan, Ltd, 9-1 Takakura-cho, Hachioji, Tokyo, 192-8510, Japan. , (Japan)
  • 4 Division of Neurogenetics, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan. , (Japan)
Type
Published Article
Journal
Analytical Biochemistry
Publisher
Elsevier
Publication Date
Jan 15, 2020
Volume
589
Pages
113508–113508
Identifiers
DOI: 10.1016/j.ab.2019.113508
PMID: 31751532
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The analysis methods for fecal short-chain fatty acids (SCFAs) have evolved considerably. Recently, the role of SCFAs in gastrointestinal physiology and their association with intestinal microbiota and disease were reported. However, the intra-fecal variability and storage stability of SCFAs have not been extensively investigated. The aim of this study was to understand the limitations of the measurement of SCFAs in crude feces and develop a useful pre-examination procedure using the freeze-drying technique. SCFAs in crude feces, obtained from healthy volunteers, and freeze-dried feces were determined by derivatization with isobutyl chloroformate, followed by liquid-liquid extraction with hexane, and separation and analysis using gas chromatography-mass spectrometry. Among the SCFAS, the maximum intra-fecal variability was observed for iso-butyrate (coefficient of variation of 37.7%), but the freeze-drying procedure reduced this variability (coefficient of variation of 7.9%). Similar improvements were also observed for other SCFAs. Furthermore, significant decreases in the SCFA amounts were observed with storage at 4 °C for 24 h. The freeze-drying procedure affords fecal SCFA stability, even with storage at room temperature for 3 d. The freeze-drying procedure allows reliable SCFA measurements without labour-intensive processes. Therefore, the freeze-drying procedure can be applied in basic, clinical, and epidemiological studies. Copyright © 2019 Elsevier Inc. All rights reserved.

Report this publication

Statistics

Seen <100 times