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Fractionation of CNBr fragments and primary structures of peptides of the adenovirus hexon protein.

Authors
Type
Published Article
Journal
The Journal of biological chemistry
Publication Date
Volume
256
Issue
12
Pages
6187–6198
Identifiers
PMID: 7240197
Source
Medline
License
Unknown

Abstract

The adenovirus hexon protein has been carboxymethylated with 14C-labeled iodoacetate. After treatment with CNBr, the peptide mixture was fractionated into fragments with seven size classes by exclusion chromatography. Large fragments were further purified by CM-cellulose chromatography in urea, and small fragments were purified by high voltage paper electrophoresis. Amino acid sequences of pure fragments have been determined by combined use of sequenator-based direct degradations, and of manual t-dimethylaminonaphthalene-1-sulfonyl-monitored Edman degradations subsequent to enzymatic redigestions. Fractionations are given, the primary structures of 22 CNBr fragments containing a total of 677 residues are reported, and the analytical aspects of the structural properties are considered.

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