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Fractionation and Analysis of Polypeptides of Euglena gracilis Chloroplasts 1

Authors
  • Aurea C. Vasconcelos
  • Leticia R. Mendiola-Morgenthaler
  • Gary L. Floyd
  • Jeffrey L. Salisbury
Publication Date
Jul 01, 1976
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

Intact Euglena gracilis chloroplasts, purified on gradients of silica sol, were lysed osmotically and fractionated by centrifugation on discontinuous gradients of sucrose into their soluble, envelope membrane, and thylakoid membrane components. The proteins of the different subchloroplast fractions, as well as those of whole chloroplasts, were analyzed by electrophoresis on sodium dodecyl sulfate polyacrylamide gels. The polypeptide profile of each fraction was distinctive and was in general similar to the profile obtained for analogous fractions of the chloroplasts of higher plants.

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