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Formycin triphosphate as a probe for the ATP binding site involved in the activation of guanylate cyclase.

Authors
Type
Published Article
Journal
European Journal of Pharmacology
0014-2999
Publisher
Elsevier
Publication Date
Volume
227
Issue
2
Pages
229–231
Identifiers
PMID: 1358664
Source
Medline
License
Unknown

Abstract

Formycin A triphosphate (FTP), a fluorescent analog of ATP, slightly increased basal guanylate cyclase activity, but significantly potentiated guanylate cyclase activity stimulated by atrial natriuretic factor (ANF) in rat lung membranes. FTP potentiated ANF-stimulated guanylate cyclase activity with an EC50 at about 90 microM and inhibited ATP-stimulated guanylate cyclase activity with an IC50 at about 100 microM. These results indicate that FTP binds more tightly than ATP for the same binding site. Therefore, FTP would be an excellent tool for studying the ATP binding site.

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