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The formation of sulphur-containing amino acids in germinating seeds of rape (Brassica napus L.).

Authors
  • Ngo, T T
  • Shargool, P D
Type
Published Article
Journal
The Biochemical journal
Publication Date
Feb 01, 1972
Volume
126
Issue
4
Pages
985–991
Identifiers
PMID: 5073247
Source
Medline
License
Unknown

Abstract

Sodium [(35)S]sulphide was fed to batches of germinating rapeseed, in some instances with the addition of unlabelled cysteine. Both the total radioactivity and specific radioactivity of the free sulphur-containing amino acids were examined. Cysteine and homocysteine were rapidly labelled; label subsequently appeared in cystathionine and methionine. The results obtained indicated that both the sulphydration and trans-sulphuration pathways were operating. This conclusion was reinforced by the results of experiments in which batches of rapeseed were incubated with l-[(14)C]homoserine. These showed the formation of labelled homocysteine, cystathione and methionine. It was thought the trans-sulphuration pathway was making the greater contribution to the biosynthesis of methionine in germinating rapeseed.

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