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Formation of embryoid bodies from mouse embryonic stem cells cultured on silicon-coated surfaces.

Authors
  • Fathi, Fardin
  • Altiraihi, Taki
  • Mowla, Seyed Javad
  • Movahedin, Mansoreh
Type
Published Article
Journal
Cytotechnology
Publication Date
Jan 01, 2009
Volume
59
Issue
1
Pages
11–16
Identifiers
DOI: 10.1007/s10616-009-9188-x
PMID: 19294527
Source
Medline
License
Unknown

Abstract

Embryoid bodies (EBs) are primitive embryonic structures derived from differentiating embryonic stem cells (ESCs). Many techniques have been used to obtain EBs. Improving the technique of EB formation can help in achieving better results in ESCs differentiation into neurons, myocardiocytes, haemopoeitic cells, and others. We evaluated the use of Sigmacote as a hydrophobic substrate to improve EB formation. CCE and P19 cell lines were used to obtain EBs and retinoic acid was used to induce neural differentiation. The results revealed that Sigmacote, as a hydrophobic substrate, can improve EB formation from ESCs. Our results demonstrate that the silicon-coating of glass petri dishes by Sigmacote is an easy and reproducible technique to enhance EB formation from murine ESCs and EC cells.

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