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Forever young: how to control the elongation, differentiation, and proliferation of cells using nanotechnology.

Authors
  • Ellis-Behnke, R G
  • Liang, Y X
  • Guo, J
  • Tay, D K C
  • Schneider, G E
  • Teather, L A
  • Wu, W
  • So, K F
Type
Published Article
Journal
Cell Transplantation
Publisher
SAGE Publications
Publication Date
Jan 01, 2009
Volume
18
Issue
9
Pages
1047–1058
Identifiers
DOI: 10.3727/096368909X471242
PMID: 20040141
Source
Medline
License
Unknown

Abstract

Within the emerging field of stem cells there is a need for an environment that can regulate cell activity, to slow down differentiation or proliferation, in vitro or in vivo while remaining invisible to the immune system. By creating a nanoenvironment surrounding PC12 cells, Schwann cells, and neural precursor cells (NPCs), we were able to control the proliferation, elongation, differentiation, and maturation in vitro. We extended the method, using self-assembling nanofiber scaffold (SAPNS), to living animals with implants in the brain and spinal cord. Here we show that when cells are placed in a defined system we can delay their proliferation, differentiation, and maturation depending on the density of the cell population, density of the matrix, and the local environment. A combination of SAPNS and young cells can be implanted into the central nervous system (CNS), eliminating the need for immunosuppressants.

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