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Fluorometric study on the interaction between lomefloxacin and bovine lactoferrin.

Authors
Type
Published Article
Journal
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
Publication Date
Volume
22
Issue
3
Pages
427–430
Identifiers
PMID: 16733316
Source
Medline

Abstract

The binding of lomefloxacin to bovine lactoferrin (BLf) in a dilute aqueous solution was studied using fluorescence spectra. The binding constant (K) and the number of binding sites (n) were obtained by a fluorescence quenching method. The binding distance (r) and energy-transfer efficiency (E) between lomefloxacin and bovine lactoferrin were also obtained according to the mechanism of Foörster-type dipole-dipole nonradiative energy-transfer. The effect of lomefloxacin on the conformation of bovine lactoferrin was also analyzed by synchronous fluorescence spectroscopy. The interaction between lomefloxacin and bovine lactoferrin is strong. Lomefloxacin can affect the conformation of bovine lactoferrin to some degree.

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