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Dietary Fiber Treatment Corrects the Composition of Gut Microbiota, Promotes SCFA Production, and Suppresses Colon Carcinogenesis.

Authors
  • Bishehsari, Faraz1
  • Engen, Phillip A2
  • Preite, Nailliw Z3
  • Tuncil, Yunus E4
  • Naqib, Ankur5
  • Shaikh, Maliha6
  • Rossi, Marco7
  • Wilber, Sherry8
  • Green, Stefan J9, 10
  • Hamaker, Bruce R11
  • Khazaie, Khashayarsha12
  • Voigt, Robin M13
  • Forsyth, Christopher B14
  • Keshavarzian, Ali15, 16, 17, 18
  • 1 Department of Internal Medicine, Division of Gastroenterology, Rush University Medical Center, Chicago, IL USA. [email protected]
  • 2 Department of Internal Medicine, Division of Gastroenterology, Rush University Medical Center, Chicago, IL USA. [email protected]
  • 3 Department of Internal Medicine, Division of Gastroenterology, Rush University Medical Center, Chicago, IL USA. [email protected]
  • 4 Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, West Lafayette, IN USA. [email protected]
  • 5 DNA Services Facility, Research Resources Center, University of Illinois at Chicago, Chicago, IL USA. [email protected]
  • 6 Department of Internal Medicine, Division of Gastroenterology, Rush University Medical Center, Chicago, IL USA. [email protected] , (Mali)
  • 7 Department of Internal Medicine, Division of Gastroenterology, Rush University Medical Center, Chicago, IL USA. [email protected]
  • 8 Department of Internal Medicine, Division of Gastroenterology, Rush University Medical Center, Chicago, IL USA. [email protected]
  • 9 DNA Services Facility, Research Resources Center, University of Illinois at Chicago, Chicago, IL USA. [email protected]
  • 10 Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL USA. [email protected]
  • 11 Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, West Lafayette, IN USA. [email protected]
  • 12 Department of Immunology, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN 55905, USA. [email protected]
  • 13 Department of Internal Medicine, Division of Gastroenterology, Rush University Medical Center, Chicago, IL USA. [email protected]
  • 14 Department of Internal Medicine, Division of Gastroenterology, Rush University Medical Center, Chicago, IL USA. [email protected]
  • 15 Department of Internal Medicine, Division of Gastroenterology, Rush University Medical Center, Chicago, IL USA. [email protected]
  • 16 Department of Physiology, Rush University Medical Center, Chicago, IL USA. [email protected]
  • 17 Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht Netherlands. [email protected] , (Netherlands)
  • 18 Department of Pharmacology, Rush University Medical Center, Chicago, IL USA. [email protected]
Type
Published Article
Journal
Genes
Publisher
MDPI AG
Publication Date
Feb 16, 2018
Volume
9
Issue
2
Identifiers
DOI: 10.3390/genes9020102
PMID: 29462896
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Epidemiological studies propose a protective role for dietary fiber in colon cancer (CRC). One possible mechanism of fiber is its fermentation property in the gut and ability to change microbiota composition and function. Here, we investigate the role of a dietary fiber mixture in polyposis and elucidate potential mechanisms using TS4Cre×cAPCl°x468 mice. Stool microbiota profiling was performed, while functional prediction was done using PICRUSt. Stool short-chain fatty acid (SCFA) metabolites were measured. Histone acetylation and expression of SCFA butyrate receptor were assessed. We found that SCFA-producing bacteria were lower in the polyposis mice, suggesting a decline in the fermentation product of dietary fibers with polyposis. Next, a high fiber diet was given to polyposis mice, which significantly increased SCFA-producing bacteria as well as SCFA levels. This was associated with an increase in SCFA butyrate receptor and a significant decrease in polyposis. In conclusion, we found polyposis to be associated with dysbiotic microbiota characterized by a decline in SCFA-producing bacteria, which was targetable by high fiber treatment, leading to an increase in SCFA levels and amelioration of polyposis. The prebiotic activity of fiber, promoting beneficial bacteria, could be the key mechanism for the protective effects of fiber on colon carcinogenesis. SCFA-promoting fermentable fibers are a promising dietary intervention to prevent CRC.

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