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Fluorescent determination of Hg2+ in water and fish samples using a chemodosimeter based in a Rhodamine 6G derivative and a portable fiber-optic spectrofluorimeter.

Authors
  • Bohoyo Gil, Diego
  • Rodriguez-Cáceres, María Isabel
  • Hurtado-Sánchez, María del Carmen
  • Muñoz de la Peña, Arsenio
Type
Published Article
Journal
Applied Spectroscopy
Publisher
SAGE Publications
Publication Date
May 01, 2010
Volume
64
Issue
5
Pages
520–527
Identifiers
PMID: 20482971
Source
Medline
License
Unknown

Abstract

A fluorimetric chemodosimeter (FC1), based on a Rhodamine 6G derivative, is proposed for the recognition of Hg(2+) ions in water and fish samples. The reagent shows a highly selective and sensitive reaction with Hg(2+), giving rise to strong fluorescence emission at 555 nm. The obvious color change of the solution from colorless to pink upon the addition of Hg(2+) demonstrates that FC1 can be used for "naked-eye" detection of Hg(2+) in water effluents. The fluorescence intensity is proportional to the amount of Hg(2+) at ng mL(-1) levels, and it is capable of distinguishing between safe and toxic levels of inorganic mercury in drinking water and fish samples. The procedure has been implemented in a portable instrument composed of a 515 nm light-emitting diode (LED) excitation source, two fiber optics, and a charge-coupled device (CCD) camera as detector, connected to a portable computer for data acquisition and analysis, intended for in situ determination of mercury, offering a viable alternative to a conventional spectrofluorimeter. The proposed method has been applied to different water and fish samples with satisfactory results.

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