Affordable Access

Fluorescence spectroscopy for revealing mechanisms in biology: Strengths and pitfalls

Authors
  • KRISHNAMOORTHY, G
Publication Date
Dec 03, 2018
Source
DSpace at IIT Bombay
Keywords
License
Unknown
External links

Abstract

This article describes the basic principles of steady-state and time-resolved fluorescence. The formal equivalence of the two methodologies is described first, followed by the extra advantages of time-resolved methods in revealing population heterogeneity in complex systems encountered in biology. Several examples from the author's work are described in support of the above contention. Finally, several misinterpretations and pitfalls in the interpretation of fluorescence data and their remedy are described.

Report this publication

Statistics

Seen <100 times