Affordable Access

Fluorescence spectra of cells stained with a DNA-specific dye, measured by flow cytometry.

Authors
Type
Published Article
Journal
Cytometry
Publication Date
Volume
7
Issue
1
Pages
104–106
Identifiers
PMID: 2419053
Source
Medline

Abstract

Fluorescence spectra of ethanol-fixed rat thymocytes stained with the DNA-specific dye Hoechst 33258 have been measured in an arc lamp-based flow cytometer including a grating monochromator in front of the fluorescence detector. Spectral resolution was 5-10 nm. Increasing dye concentration was found to yield an increasing shift of the fluorescence spectrum toward longer wavelengths, thus supporting previous work on soluble DNA that indicated several different binding modes of this dye. The results show that similar data may be obtained for all commonly used DNA-specific dyes. It appears that this type of spectral information may be used to probe the structure of cell chromatin.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments