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Fluorescence in situ hybridization in plants: recent developments and future applications.

Authors
  • Jiang, Jiming1
  • 1 Department of Plant Biology, Department of Horticulture, Michigan State University, East Lansing, MI, 48824, USA. [email protected]
Type
Published Article
Journal
Chromosome Research
Publisher
Springer-Verlag
Publication Date
Sep 01, 2019
Volume
27
Issue
3
Pages
153–165
Identifiers
DOI: 10.1007/s10577-019-09607-z
PMID: 30852707
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Fluorescence in situ hybridization (FISH) was developed more than 30 years ago and has been the most paradigm-changing technique in cytogenetic research. FISH has been used to answer questions related to structure, mutation, and evolution of not only individual chromosomes but also entire genomes. FISH has served as an important tool for chromosome identification in many plant species. This review intends to summarize and discuss key technical development and applications of FISH in plants since 2006. The most significant recent advance of FISH is the development and application of probes based on synthetic oligonucleotides (oligos). Oligos specific to a repetitive DNA sequence, to a specific chromosomal region, or to an entire chromosome can be computationally identified, synthesized in parallel, and fluorescently labeled. Oligo probes designed from conserved DNA sequences from one species can be used among genetically related species, allowing comparative cytogenetic mapping of these species. The advances with synthetic oligo probes will significantly expand the applications of FISH especially in non-model plant species. Recent achievements and future applications of FISH and oligo-FISH are discussed.

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