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Fluorescence properties, thermal duplex stability, and kinetics of formation of cyanin platinum DNAs.

Authors
  • van de Rijke, Frans M
  • Heetebrij, Robert J
  • Talman, Eduard G
  • Tanke, Hans J
  • Raap, Anton K
Type
Published Article
Journal
Analytical biochemistry
Publication Date
Oct 01, 2003
Volume
321
Issue
1
Pages
71–78
Identifiers
PMID: 12963057
Source
Medline
License
Unknown

Abstract

Fluorescent and haptenized, monofunctionally binding platinum compounds are increasingly used for chemically labeling nucleic acids for hybridization detection purposes. Here we analyze in detail the effect of labeling density of the cyanin-3 and -5 platinum DNA adducts on fluorescence and thermal stability. We also analyzed the kinetics of the reaction of the cyanin platinum compounds with DNA. The data provided are important for the design of optimal platinum DNA labeling and hybridization conditions for fluorescence hybridization applications.

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