Affordable Access

Fluorescence photobleaching analysis of nuclear transport: dynamic evidence for auxiliary channels in detergent-treated nuclei.

Authors
Type
Published Article
Journal
Biochemistry
Publication Date
Volume
26
Issue
6
Pages
1546–1551
Identifiers
PMID: 2439114
Source
Medline
License
Unknown

Abstract

Nuclear transport experiments were performed on isolated rat liver nuclei to examine the permeability of membrane and detergent-free peripheral nuclear lamina. The transport of 64K molecular weight fluorescent-derivatized dextrans was measured by using the technique of fluorescence redistribution after photobleaching. Results of these experiments provide evidence for transport pathways that appear to be functionally distinct from nuclear pore complex channels. The suggestion is made that these supplemental pathways are embedded in the peripheral nuclear lamina and are normally masked by the inner nuclear membrane.

Statistics

Seen <100 times