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Fluorescence lifetime and quenching studies on some interesting diphenylhexatriene membrane probes

Authors
  • Cranney, Moira
  • Cundall, Robert B.
  • Jones, Gareth R.
  • Richards, John T.
  • Thomas, Emrys W.
Type
Published Article
Journal
Biochimica et Biophysica Acta (BBA) - Biomembranes
Publisher
Elsevier
Publication Date
Jan 01, 1983
Volume
735
Issue
3
Pages
418–425
Identifiers
DOI: 10.1016/0005-2736(83)90156-6
Source
Elsevier
Keywords
License
Unknown

Abstract

The fluorescence lifetimes of a number of membrane probes based on the 1,6-diphenylhexatriene (DPH) chromophore have been measured in small unilamellar phospholipid vesicles and found to be multiphasic. These probes were quenched by sodium iodide with different efficiencies in vesicles and this has been attributed to the depth of the particular probe in the bilayer. The distribution of the probe between the outer and inner monolayer has been determined for those probes with fixed positions in the bilayer. The iodide ion permeability of the bilayer was found to be immeasurably small over a 3 h period.

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