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Fluorescence lifetime-based discrimination and quantification of cellular DNA and RNA with phase-sensitive flow cytometry.

Authors
  • 1
  • 1 Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA. , (Mexico)
Type
Published Article
Journal
Cytometry. Part A : the journal of the International Society for Analytical Cytology
Publication Date
Volume
52
Issue
1
Pages
46–55
Identifiers
PMID: 12596251
Source
Medline
License
Unknown

Abstract

The lifetimes of the nucleic acid-binding fluorochromes were altered when binding to different macromolecules under different conditions. Phase-sensitive flow cytometry provided a unique means for simultaneous discrimination and quantification of subcellular macromolecules with one fluorescent probe. The data demonstrated the capabilities for resolving relative cellular DNA and RNA contents based on fluorescence lifetime.

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