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Fluorescence excitation on monolithically integrated all-polymer chips.

Authors
  • Schelb, Mauno1
  • Vannahme, Christoph
  • Welle, Alexander
  • Lenhert, Steven
  • Ross, Benjamin
  • Mappes, Timo
  • 1 Karlsruhe Institute of Technology, Institut fur Mikrostrukturtechnik, Karlsruhe, Germany. [email protected] , (Germany)
Type
Published Article
Journal
Journal of Biomedical Optics
Publisher
SPIE - International Society for Optical Engineering
Publication Date
Jan 01, 2010
Volume
15
Issue
4
Pages
41517–41517
Identifiers
DOI: 10.1117/1.3446672
PMID: 20799795
Source
Medline
License
Unknown

Abstract

All-polymer chips with integrated optical waveguides and microfluidic channels were built as a platform for fluorescence excitation of biological samples. Their functionality has been shown for four cases: (i) fluorescence of labeled phospholipids inside a microfluidic channel, (ii) fluorescence of stained cells inside a microfluidic channel, (iii) fluorescence of labeled phospholipids on top of a polymer waveguide, and (iv) fluorescence of stained cells on top of a polymer waveguide.

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