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Fluorescence confocal laser scanning microscopy for in vivo imaging of epidermal reactions to two experimental irritants.

Authors
Type
Published Article
Journal
Skin Research and Technology
1600-0846
Publisher
Wiley Blackwell (Blackwell Publishing)
Publication Date
Volume
14
Issue
4
Pages
498–503
Identifiers
DOI: 10.1111/j.1600-0846.2008.00323.x
PMID: 18937788
Source
Medline
License
Unknown

Abstract

This preliminary study demonstrated the applicability of fluorescence CLSM for a detailed study of experimental skin irritants in vivo. Essential findings were disturbed and widened cell borders, swelling of keratinocytes by PA and induction of a parakeratotic shift by SLS with clusters of keratinocytes holding nuclei in the epidermis. Fluorescence CLSM offers a unique opportunity to study the inter- and intracellular water compartments directly in the epidermis in situ and an opportunity to visualize cell proliferation manifested as parakeratosis. Fibre-optic fluorescence CLSM of irritant reactions is, however, technically more complicated than reflectance CLSM and may not be applicable to any irritant. SLS applied epicutaneously interacted with the skin surface and coupling to the microscope and was thus found to be more difficult to study technically than PA. PA dissolved in isopropanol is for technical reasons, and with SLS as alternative, considered the preferred model irritant.

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