Affordable Access

Access to the full text

FLT3-ITD DNA allelic burden, but not mRNA levels, influences the biological characteristics of AML patients

Authors
  • Soare, Dan S.1, 2,
  • Radu, Eugen2, 1
  • Dumitru, Ion1, 2
  • Arghir, Aurora2, 3
  • Enache, Cristina1, 2
  • Bumbea, Horia1, 2
  • Vlădăreanu, Ana M.1, 2
  • 1 University Emergency Hospital Bucharest, Romania , (Romania)
  • 2 Carol Davila University of Medicine and Pharmacy, Romania , (Romania)
  • 3 Victor Babeş National Institute of Pathology, Romania , (Romania)
Type
Published Article
Journal
Revista Romana de Medicina de Laborator
Publisher
De Gruyter Open Sp. z o.o.
Publication Date
Jan 01, 2021
Volume
29
Issue
1
Pages
33–41
Identifiers
DOI: 10.2478/rrlm-2021-0004
Source
De Gruyter
Keywords
License
Green

Abstract

FMS-like tyrosine kinase 3 gene internal tandem (FLT3-ITD) mutations represent one of the most frequent genetic lesions in acute myeloid leukemia (AML) and imparts a negative prognostic. For an optimal patient management, current clinical guidelines recommend the evaluation of the allelic ratio (AR), expressed as the DNA FLT3-ITD/WT mutational burden. We sought to evaluate the differences between the AR and FLT3-ITD/WT mRNA ratio (RR) and their respective impact on the biological characteristics of AML patients. A total of 32 DNA and mRNA samples from AML patients with FLT3-ITD were evaluated. There was a good correlation between the AR and RR (Spearman’s rho= 0.652, P <0.001). None of the biological characteristics were influenced by the RR values, whereas patients with high AR values (≥0.5) had higher WBC counts (Mann-Whitney, P= 0.01), LDH levels (Mann-Whitney, P= 0.037), and circulating blasts levels (Mann-Whitney, P= 0.023) than patients with low AR values (<0.5). Also, there was a good correlation between AR values and WBC count (Spearman’s correlation, P= 0.001), and LDH levels (Spearman’s correlation, P= 0.007). In our study population the AR, but not the RR, influenced the biological characteristic of patients suggesting a dose-independent effect of FLT3-ITD mutations.

Report this publication

Statistics

Seen <100 times