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Flow Cytometry Assessment of Microalgae Physiological Alterations under CO2 Injection.

Authors
  • Galotti, Andréa1, 2
  • Jiménez-Gómez, Francisco1, 2
  • Parra, Gema1, 2
  • 1 Department of Animal Biology, Plant Biology and Ecology, University of Jaén, Jaén, Spain. , (Spain)
  • 2 Centre of Advanced Studies in Earth Sciences, University of Jaén, Jaén, Spain. , (Spain)
Type
Published Article
Journal
Cytometry Part A
Publisher
Wiley (John Wiley & Sons)
Publication Date
Nov 01, 2020
Volume
97
Issue
11
Pages
1136–1144
Identifiers
DOI: 10.1002/cyto.a.24028
PMID: 32427422
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Growth rate (GR), esterase activity (EA), membrane potential (MP), and DNA content were measured by flow cytometry to test if this powerful tool could be included in risk assessment and monitoring programs. This study tests a battery of endpoints that were measured on Scenedesmus (Acutodesmus) obliquus as model species, under high levels of injected CO2 to be proposed as biomarkers of effect. New technologies such as carbon capture and storage (CCS) bring positive consequences on CO2 mitigation strategies but also could have negative consequences if a CO2 leakage occurs during injection. Under this scenario, pH might drop down to 4 pH units around the pipe where the leakage happens. The study focus on to ascertain if the endpoints fulfill the requirements such as sensitiveness, be timely and cost-effective, be easy to measure and interpret, and be nondestructive, valuable attributes in effective biomarkers. CO2 injected at high levels significantly affects the EA, the membrane polarization, as well as GRs. However the DNA content did not shown a clear response under this condition. In conclusion, the GR, the EA, and the MP analyzed by flow cytometry fulfilled the attributes mentioned above and are proposed as biomarkers of effect in CCS monitoring programs. © 2020 International Society for Advancement of Cytometry. © 2020 International Society for Advancement of Cytometry.

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