Flow cytometric printing of double emulsions into open droplet arrays.
School of Mechanical Engineering and Automation, Beihang University, Beijing, China.
Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA, USA. [email protected].
California Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, CA, USA.
- Published Article
Lab on a Chip
The Royal Society of Chemistry
- Publication Date
May 16, 2023
Delivery of double emulsions in air is crucial for their applications in mass spectrometry, bioanalytics, and material synthesis. However, while methods have been developed to generate double emulsions in air, controlled printing of double emulsion droplets has not been achieved yet. In this paper, we present an approach for in-air printing of double emulsions on demand. Our approach pre-encapsulates reagents in an emulsion that is reinjected into the device, and generates double emulsions in a microfluidic printhead with spatially patterned wettability. Our device allows sorting of ejected double emulsion droplets in real-time, allowing deterministic printing of each droplet to be selected with the desired inner cores. Our method provides a general platform for building printed double emulsion droplet arrays of defined composition at scale.
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This record was last updated on 08/06/2023 and may not reflect the most current and accurate biomedical/scientific data available from NLM.
The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/37070963