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Fixed-time artificial insemination protocols on brazilian locally adapted breed gilts on ovulatory response and embryo production

Authors
  • Silva, Priscilla Cristine Passoni1
  • Brasil, Oscar Oliveira1
  • Souto, Paula Lorena Grangeira1
  • Moreira, Nathalia Hack1
  • da Silva, Joseane Padilha2
  • Silva, Bianca Damiani Marques2
  • Ramos, Alexandre Floriani2
  • 1 Departamento de Ciências Animais, Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília, Brasília, DF, Brasil
  • 2 Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brasil
Type
Published Article
Journal
Animal Reproduction
Publisher
Colégio Brasileiro de Reprodução Animal
Publication Date
May 28, 2021
Volume
18
Issue
1
Identifiers
DOI: 10.1590/1984-3143-AR2020-0776
PMID: 34122655
PMCID: PMC8189352
Source
PubMed Central
Keywords
Disciplines
  • Original Article
License
Unknown

Abstract

The aim of this study was to use estrus synchronization protocols to favor fixed-time artificial insemination and consequently fixed-time embryo collection, and increase embryo production using eCG, in gits. In a cross over design, nine Piau breed gilts were subjected to 18 days of oral progesterone; P4 group did not receive any further; GnRH group received 25µg of GnRH 104 hours after the final application of P4; and eCG+GnRH group received 1000IU of eCG 24 hours after the final P4 in addition to GnRH for subsequent embryo collection, that was performed six days after first AI, by laparotomy. Artificial insemination was performed after 12 and 24 hours of estrus in P4 group, and 128 and 144 hours in GnRH and eCG+GnRH groups. The number of CL (8.6±3.9; 8.3±2.1; 26.7±15.0) and anovulatory follicles (4.3±3.7; 3.9±3.9; 17.2±9.5) was higher in the eCG+GnRH gilts ( P <0.05). However, the use of 1000 IU of eCG reduced ( P <0.05) the number of total structures (5.2±3.6; 5.1±3.1; 1.7±2.7), viable embryos (5.0±3.5; 4.8±3.3; 0.4±0.7), freezable embryos (3.6±3.4; 3.3±3.8; 0.1±0.3) and recovery rate (63.7±38.9; 58.6±24.7; 5.38±9.5). P4 and GnRH protocols were effective in the production and recovery of embryos. However, the use of 1000 IU of eCG, 24 hours after P4, was not effective in promoting the production of embryos, although the animals had superovulated.

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