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Fish oil alleviates circadian bile composition dysregulation in male mice with NAFLD.

Authors
  • Liu, Yang1
  • Li, Qi1
  • Wang, Hualin2
  • Zhao, Xiuju1
  • Li, Na1
  • Zhang, Hongyu1
  • Chen, Guoxun3
  • Liu, Zhiguo4
  • 1 Hubei Province Engineering Research Center of Healthy Food, School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China. , (China)
  • 2 Hubei Province Engineering Research Center of Healthy Food, School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China. Electronic address: [email protected] , (China)
  • 3 Department of Nutrition, University of Tennessee at Knoxville, Knoxville, TN, United States. , (United States)
  • 4 Hubei Province Engineering Research Center of Healthy Food, School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
The Journal of nutritional biochemistry
Publication Date
Jul 01, 2019
Volume
69
Pages
53–62
Identifiers
DOI: 10.1016/j.jnutbio.2019.03.005
PMID: 31055233
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Our previous studies have found that fish oil rich in ω-3 polyunsaturated fatty acids (ω-3 PUFA) protects against non-alcoholic fatty liver disease (NAFLD) in mice. This study was aimed to explore the effects of fish oil on high fat diet (HFD)-induced circadian bile composition chaos. Male C57BL/6 mice were randomly divided into three groups, a control group (CON), a HFD group and a fish oil (FO) group, which were fed a normal chow diet, a HFD, and a HFD supplemented with FO, respectively for 12 weeks. At the end of the experiment, liver tissue, blood and bile samples were processed at 12-h intervals with the first one at zeitgeber time 0 (ZT0) and the second at zeitgeber time 12 (ZT12). Metabolites in bile were determined using UPLC-QTOF-MS, screened using multivariate statistical analysis, and analyzed using KEGG database and Metaboanalyst. The expression levels of key proteins in bile acid metabolism were examined using western blot. Results of biochemical analysis and H&E staining illustrated that feeding of HFD induced NAFLD, which was ameliorated in FO group. The bile content of each group at ZT0 (CON, HFD, or FO group) was respectively higher than that at ZT12 (P<.05). The metabolic pathway analysis of differential metabolites showed that these differences were correlated with amino acid metabolism, fatty acid biosynthesis and primary bile acid synthesis at ZT0. FO supplement could modify bile composition, which was related to the influence of its ω-3 PUFA on liver metabolism. ω-3 PUFA may also regulate the circadian rhythm of bile metabolism. Copyright © 2019 Elsevier Inc. All rights reserved.

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