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First Metagenomic Survey of the Microbial Diversity in Bioaerosols Emitted in Waste Sorting Plants.

Authors
  • Degois, Jodelle1
  • Clerc, Frederic1
  • Simon, Xavier1
  • Bontemps, Cyril2
  • Leblond, Pierre2
  • Duquenne, Philippe1
  • 1 Department of pollutant metrology, Institut National de Recherche et de Sécurité (INRS), 1 rue du Morvan, Vandœuvre-lès Nancy 54500, France. , (France)
  • 2 Faculté des Sciences et Technologies, Institut National de la Recherche Agronomique, Dynamique des Génomes et Adaptation Microbienne (DynAMic), Université de Lorraine, UMR INRA 1128, Boulevard des Aiguillettes, BP 70239, F-54506 Vandoeuvre Cedex, France. , (France)
Type
Published Article
Journal
Annals of work exposures and health
Publication Date
Nov 10, 2017
Volume
61
Issue
9
Pages
1076–1086
Identifiers
DOI: 10.1093/annweh/wxx075
PMID: 29136413
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Waste sorting activities are source of occupational bioaerosol exposures that are associated with several health disorders. New analytical tools, based on next-generation sequencing (NGS) technologies, provide powerful methods to assess the microbial composition of bioaerosols. The objectives of the study were (i) to assess the feasibility and the repeatability of NGS-based biodiversity measurements and (ii) to study the microbial biodiversity using NGS in bioaerosols emitted in a waste sorting plant (WSP). Three stationary parallel samples were collected in a sorting cabin using closed-face cassettes equipped with polycarbonate membranes. Bacterial and fungal diversity was assessed by sequencing 16S and 18S rDNA genes using either Illumina sequencing or 454 pyrosequencing methods. At sampling point, airborne bacteria were dominated by Proteobacteria, Firmicutes, and Actinobacteria with prevailing genera assigned to unclassified Enterobacteriaceae, Staphylococcus, Acinetobacter, Leuconostoc, Pseudomonas, and Lactobacillus. Airborne fungi were dominated by Ascomycota with prevailing genera assigned to Penicillium, Aspergillus, Rhizopus, Wallemia, and Hemicarpenteles. The NGS biodiversity measurements revealed a higher biodiversity bioaerosols that previously reported for WSP in studies carried out using culture methods followed by identification of microorganisms. These results provide the first survey about taxonomic biodiversity in bioaerosols from WSPs using high-throughput sequencing. © The Author 2017. Published by Oxford University Press on behalf of the British Occupational Hygiene Society.

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