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First metagenomic survey of the microbial diversity in bioaerosols emitted in waste sorting plants

Authors
  • Degois, Jodelle
  • Clerc, Frédéric
  • Simon, Xavier
  • Bontemps, Cyril
  • Leblond, Pierre
  • Duquenne, Philippe
Publication Date
Sep 01, 2017
Source
HAL-UPMC
Keywords
Language
English
License
Unknown
External links

Abstract

Waste sorting activities are source of occupational bioaerosol exposures that are associated with several health disorders. New analytical tools, based on next generation sequencing (NGS) technologies, provide powerful methods to assess the microbial composition of bioaerosols. The objectives of the study were (1) to assess the feasibility and the repeatability of NGS based biodiversity measurements and (2) to study the microbial biodiversity using NGS in bioaerosols emitted in a waste sorting plant (WSP). Three stationary parallel samples were collected in a sorting cabin using closed-face cassettes equipped with polycarbonate membranes. Bacterial and fungal diversity was assessed by sequencing 16S and 18S rDNA genes using either Illumina sequencing or 454 pyrosequencing methods. At sampling point, airborne bacteria were dominated by Proteobacteria, Firmicutes and Actinobacteria with prevailing genera assigned to unclassified Enterobacteriaceae, Staphylococcus, Acinetobacter, Leuconostoc, Pseudomonas and Lactobacillus. Airborne fungi were dominated by Ascomycota with prevailing genera assigned to Penicillium, Aspergillus, Rhizopus,

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