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First Experimental Assessment of Protein Intrinsic Disorder Involvement in an RNA Virus Natural Adaptive Process.

Authors
  • Charon, Justine1, 2
  • Barra, Amandine1
  • Walter, Jocelyne1
  • Millot, Pauline3
  • Hébrard, Eugénie4
  • Moury, Benoît3
  • Michon, Thierry1
  • 1 UMR Biologie du Fruit et Pathologie, INRA, Université de Bordeaux, Villenave d'Ornon, France. , (France)
  • 2 CNRS 5320, INSERM U1212, Pessac, France. , (France)
  • 3 UR Pathologie Végétale, INRA, Montfavet, France. , (France)
  • 4 UMR Interactions Plantes-Microorganismes-Environnement, IRD, CIRAD, Université de Montpellier, Montpellier, France. , (France)
Type
Published Article
Journal
Molecular Biology and Evolution
Publisher
Oxford University Press
Publication Date
Jan 01, 2018
Volume
35
Issue
1
Pages
38–49
Identifiers
DOI: 10.1093/molbev/msx249
PMID: 29029259
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Intrinsic disorder (ID) in proteins is defined as a lack of stable structure in physiological conditions. Intrinsically disordered regions (IDRs) are highly abundant in some RNA virus proteomes. Low topological constraints exerted on IDRs are expected to buffer the effect of numerous deleterious mutations and could be related to the remarkable adaptive potential of RNA viruses to overcome resistance of their host. To experimentally test this hypothesis in a natural pathosystem, a set of four variants of Potato virus Y (PVY; Potyvirus genus) containing various ID degrees in the Viral genome-linked (VPg) protein, a key determinant of potyvirus adaptation, was designed. To estimate the ID contribution to the VPg-based PVY adaptation, the adaptive ability of the four PVY variants was monitored in the pepper host (Capsicum annuum) carrying a recessive resistance gene. Intriguingly, the two mutants with the highest ID content displayed a significantly higher ability to restore infection in the resistant host, whereas the less intrinsically disordered mutant was unable to restore infection. The role of ID on virus adaptation may be due either to a larger exploration of evolutionary pathways or the minimization of fitness penalty caused by resistance-breaking mutations. This pioneering study strongly suggests the positive impact of ID in an RNA virus adaptive capacity. © The Author 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

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